FY-EH4609 Human f-Hb(Free Haemoglobin) ELISA Kit

FeiyueBio a speciality supplier in research kits, antibodies and reagents for life scientists working in biotech, pharma and academia. Our platform offers an extensive range of research materials for the detection, qualification and quantification of proteins and metabolites.

Synonyms: Hgb; Haemoglobin; Heterotetramer(αβ)2

f-Hb(Free Haemoglobin) Basic information

Hemoglobin, (haemoglobin BrE) (from the Greek word αἷμα, haîma 'blood' Latin globus 'ball, sphere' -in) (/ˌhiːməˈɡloʊbɪn, ˈhɛmoʊˌ-/), abbreviated Hb or Hgb, is the iron-containing oxygen-transport metalloprotein in red blood cells (erythrocytes) of almost all vertebrates (the exception being the fish family Channichthyidae) as well as the tissues of some invertebrates. Hemoglobin in blood carries oxygen from the respiratory organs (e.g. lungs or gills) to the rest of the body (i.e. tissues). There it releases the oxygen to permit aerobic respiration to provide energy to power functions of an organism in the process called metabolism. A healthy individual human has 12 to 20 grams of hemoglobin in every 100 mL of blood.

Human f-Hb(Free Haemoglobin) ELISA Kit test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.  

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