Hemoglobin / Transferrin Joint Detection the Test Kit

Principle】

This product was developed by double antibody sandwich method, respectively, in the detection zone on the nitrocellulose membrane coated with anti-hemoglobin and transferrin monoclonal antibody and in the control area coated goat anti-mouse IgG. In the assay, the hemoglobin and transferrin antigens in the sample can be bound to the colloidal gold-antibody coated on the front of the reagent to form an immunocomplex. Such as positive samples, respectively, in the detection zone and the control area of ​​the aggregation of the formation of a ribbon; the case of negative samples, only the control area to form a ribbon.

[Intended use]
The product is used for in vitro qualitative detection of human hemoglobin and transferrin in the content.
Healthy daily gastrointestinal physiological blood loss of 0.6ml, greater than 2ml that is pathological bleeding. Hemoglobin and transferrin in the blood ratio of 51.2: 1, and fecal hemoglobin and transferrin ratio of 5.4: 1 [1], about 40-50% of patients with upper gastrointestinal bleeding can not be hemoglobin immunoassay (1) hemoglobin or red blood cells after digestive enzyme degradation or digestion depleted, already does not have the original activity; (2) excessive hemoglobin led to the reaction system of antigen excess lag phenomenon; ③ patients with hemoglobin antigen and monoclonal antibody match.
Transferrin is a beta globulin, present in the plasma, when the gastrointestinal bleeding, plasma transferrin will leak into the gastrointestinal tract, and excreted with the feces in vitro [2], when people in the sample transferrin content Up to 40ng / ml can be detected.
Transferrin is more stable in the digestive tract than hemoglobin. In the detection of hemoglobin at the same time, the detection of transferrin, can greatly reduce the occurrence of false negative response, improve the positive detection rate [3-4].