Human FLOT1(Flotillin-1) ELISA Kit test method

Feiyue’s Human FLOT1 Elisa kit is an ELISA reagent for detection of Neutrophil elastase in Tissue homogenates, cell lysates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of  FLOT1(Flotillin-1) . FLOT1(Flotillin-1) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti- FLOT1(Flotillin-1) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of  FLOT1(Flotillin-1) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1."Flotillin-1: gene structure: cDNA cloning from human lung and the identification of alternative polyadenylation signals."

2."Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34 hematopoietic stem/progenitor cells."

3."Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region."

4."Complete sequencing and characterization of 21,243 full-length human cDNAs."