Human IL-6(Interleukin 6) ELISA Kit Basic Information 

Feiyue’s Human IL-6 is an ELISA reagent for detection of IL-6 in human serum, plasma or cell with sensitivity, specificity and consistency.

Human IL-6(Interleukin 6) Introduciton

Interleukin-6, abbreviated as interleukin-6, was originally discovered by the team of Japanese immunologist Chuzo Kishimoto. It is an interleukin that mediates inflammation (but in some cases it also has an anti-inflammatory effect). Interleukin-6 can be secreted by T cells, B cells, monocytes, fibroblasts, keratinocytes, endothelial cells, mesangial cells, and some tumor cells. Interleukin-6 was originally thought to be a factor that can promote the differentiation of B cells into plasma cells, but other functions have been discovered later. For example, interleukin-6 can cross the blood-brain barrier, which is related to the normal physiological process of the central nervous system and the process of damage repair.

Human IL-6(Interleukin 6) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human IL-6.

Human IL-6-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-6 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IL-6 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000136244 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000025746 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.