Human LH(Luteinizing Hormone) ELISA Kit

LH(Luteinizing Hormone) Basic information

Luteinizing hormone (LH, also known as lutropin and sometimes lutrophin) is a hormone produced by gonadotropic cells in the anterior pituitary gland. The production of LH is regulated by gonadotropin-releasing hormone (GnRH) from the hypothalamus. In females, an acute rise of LH ("LH surge") triggers ovulation and development of the corpus luteum. In males, where LH had also been called interstitial cell–stimulating hormone (ICSH), it stimulates Leydig cell production of testosterone. It acts synergistically with follicle-stimulating hormone (FSH).

Human LH(Luteinizing Hormone) ELISA Kit test method

Feiyue’s Human LH(Luteinizing Hormone) is an ELISA reagent for detection of LH(Luteinizing Hormone) in Serum, plasma and other biological fluids.

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Feiyuebio May promotion for research kit: ELISA, Antibody, protein.
[email protected]

Reference:

1.Ujihara M, Yamamoto K, Nomura K, Toyoshima S, Demura H, Nakamura Y, et al. (June 1992). "Subunit-specific sulphation of oligosaccharides relating to charge-heterogeneity in porcine lutrophin isoforms". Glycobiology. 2 (3): 225–31. doi:10.1093/glycob/2.3.225. PMID 1498420.

2.Stamatiades, George A.; Kaiser, Ursula B. (2018-03-05). "Gonadotropin regulation by pulsatile GnRH: Signaling and gene expression". Molecular and Cellular Endocrinology. Signaling Pathways Regulating Pituitary Functions. 463: 131–141. doi:10.1016/j.mce.2017.10.015. ISSN 0303-7207. PMC 5812824. PMID 29102564.