Human FUS(RNA-binding protein FUS) ELISA Kit

FUS(RNA-binding protein FUS) Basic information

RNA-binding protein FUS/TLS (FUused in Sarcoma/Translocated in LipoSarcoma), also known as heterogeneous nuclear ribonucleoprotein P2 is a protein that in humans is encoded by the FUS gene. The N-terminal end of FUS appears to be involved in transcriptional activation, while the C-terminal end is involved in protein and RNA binding. In addition recognition sites for the transcription factors AP2, GCF, Sp1 have been identified in FUS. Consistently, in vitro studies have shown that FUS/TLS binds RNA, single-stranded DNA and (with lower affinity) double-stranded DNA. The sequence specificity of FUS/TLS binding to RNA or DNA has not been well established; however, using in vitro selection (SELEX), a common GGUG motif has been identified in approximately half of the RNA sequences bound by FUS/TLS.

Human FUS(RNA-binding protein FUS) ELISA Kit test method

Feiyue’s Human FUS(RNA-binding protein FUS) is an ELISA reagent for detection of FUS(RNA-binding protein FUS) in tissue homogenates, cell lysates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Human FUS(RNA-binding protein FUS). Human FUS(RNA-binding protein FUS)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human FUS(RNA-binding protein FUS) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human FUS(RNA-binding protein FUS) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000089280 - Ensembl, May 2017

2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

3. Eneroth M, Mandahl N, Heim S, Willén H, Rydholm A, Alberts KA, Mitelman F (Aug 1990). "Localization of the chromosomal breakpoints of the t(12;16) in liposarcoma to subbands 12q13.3 and 16p11.2". Cancer Genet Cytogenet. 48 (1): 101–7. doi:10.1016/0165-4608(90)90222-V. PMID 2372777.